Transcriptomic and proteomic analysis of putative digestive proteases in the salivary gland and gut of Empoasca (Matsumurasca) onukii Matsuda

Abstract Background Infestation by tea green leafhoppers ( Empoasca (Matsumurasca) onukii ) can cause a series of biochemical changes in leaves. As typical cell-rupture feeder, E. secretes proteases while using its stylet to probe the tender shoots plants Camellia sinensis ). This study identified and analyzed expressed specifically salivary gland (SG) gut through enzymatic activity assays complemented with an integrated analysis transcriptomic proteomic data. Results In total, 129 contigs representing seven types putative were identified. Transcript abundance digestive showed that cathepsin B-like protease, L-like serine (trypsin- chymotrypsin-like protease) highly abundant but moderately SG. The pattern SG differed from other hemipterans, including Nilaparvata lugens , Laodelphax striatellus Acyrthosiphum pisum Halyomorpha halys Nephotettix cincticeps . Phylogenetic aminopeptidase N-like proteins or hemipterans formed two distinct clusters. Conclusions Altogether, this provides insightful information on system Compared five hemipteran species, we observed different patterns These results will be beneficial understanding interaction between